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The fluorescent proteins are designed co-expressed with the target to monitor transfection and expression efficiencies. The SmartBac system integrates the univector plasmid-fusion system, Gibson assembly method and polyprotein strategy to construct the final transfer plasmid.


Here we report SmartBac, an easy and versatile system for constructing large-sized transfer plasmids used to generate recombinant baculoviruses that express large multiprotein complexes in insect cells.
ADD PIECES IN TO PLASMID MAPS SNAPGENE VIEWER HOW TO
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